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Endotoxins - Evaluation of a Semi-Automated Endotoxin Testing Workflow

Using a Recombinant Factor C (rFC) Assay and an Automated Pipetting Robot

The ENDOZYME® II GO was recently developed including a 96-well microplate pre-loaded with required Control Standard Endotoxin (CSE) amounts for the standard curve as well as Positive Product Controls (PPC). In order to minimize handling time and further optimize the assay workflow, without the need for dedicated personnel, a semi-automated solution including an automated pipetting robot has now been assessed. The semi-automated workflow consists of three main parts; rFC reagents including the microplate pre-loaded with CSE, an INTEGRA ASSIST PLUS pipetting robot and a BioTek fluorescence microplate reader.

 

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