ESTREAM^®

Automated PCR setup from easyMAG® eluates

The ESTREAM® instrument is a compact pipetting instrument for any laboratory requiring fully automated setup of real-time PCR amplification reactions and sample traceability.

The system can prepare a 96-well PCR reaction plate from nucleic acid eluates in either easyMAG vessels or storage tubes, or a combination of both, in ~35 minutes

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Overview

ESTREAM Overview

The ESTREAM instrument is a compact pipetting instrument for any laboratory requiring fully automated setup of real-time PCR amplification reactions and sample traceability. The system can prepare a 96-well PCR reaction plate from master mixes and nucleic acid eluates presented in either easyMAG vessels or storage tubes, or from both, in about 35 minutes.

ESTREAM is designed to prepare multiple quantitative and qualitative real-time PCR assays. Intelligent pipetting from a test request file allows processing of samples requiring varying subsets of master mixes without re-programming the instrument.

The system includes a barcode reader, UV light for decontamination, and provides a positive pressure HEPA-filtered atmosphere.

Uses

ESTREAM Uses

ESTREAM is designed to prepare multiple quantitative and qualitative real-time PCR assays.
Enables more productivity from your personnel
Less demanding workflow for your staff
Frees prep time that can get the thermocycler started earlier
Provides traceability to support accreditation and peace of mind

Supported thermocyclers and associated consumables
Life Technologies (ABI)	7500: MicroAmp® Optical 96-Well reaction Plate 7500: FastMicroAmp® Fast Optical 96-Well Reaction Plate
Bio-Rad Laboratories	CFX96 Real-Time: Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates
Roche	LightCycler 480 II: LightCycler® 480 Multiwell Plate 96, white
QIAGEN	Rotor-Gene Q: Rotor-Disc 72, Strip Tubes and Caps, 0.1 ml, PCR Tubes, 0.2 ml

Features

ESTREAM Features

Combine multiple quantitative and/or qualitative real-time assays per plate
Supports frequently-used thermocycler consumables from ABI, Roche, BioRad and Qiagen
Software that thinks like you do: combine samples plus master mixes to create reactions
Intelligent pipetting from .csv files: daily workflow involves no re-programming
Flexibility to run commercial assays and your laboratory-developed tests
Magnetic racks hold easyMAG eluates stable during pipetting
UV sterilization: UV light system for desk sterilization under closed lid conditions
HEPA-Filter: High Efficiency Particulate Air Filter system provides positive pressure
Cooled Reagent and PCR Plate Holders

Specifications

Compact benchtop automated liquid handler
23.6” W x 20.3” D x 18.5” H
Disposable aerosol-barrier tips, 50 μl and 200 μl
5 - 190 μl pipetting range
Single channel, air filled
Uses magnetic racks that hold ESTREAM eluates stable during pipetting.
Up to 6 easyMAG vessels = 48 input eluates/per plate (in standard configuration)
24 passively-cooled positions for master mixes or controls
Cooled position for PCR reaction plate
Additional 64 tube positions for additional input elutes or destination storage tubes
Supports frequently-used thermocycler consumables from ABI, Roche, BioRad and Qiagen

Parameters	ESTREAM
Year Introduced to Market	2014 (Europe); 2015 (US)
Technology Type	Air based pipetting system + pLLS (pressure Liquid Level Sensing)
Instrument Footprint (W x D x H)	Width = 600 mm - Depth = 515 mm - Height = 470 mm
Weight	45 Kg
Operating Ambient Humidity	Relative humidity : 20 to 80%
Operating Temperature Range	18 to 25°C
Electrical	100-250 VAC, 50/60 Hz, 60 watts
Maximum Altitude Installation	1000 m
Noise Output in Decibels (dB)	42,5 dB(A)
Table Capacity	9x microplates full positions and 3 reagent racks positions
Pipetting head	Single channel , exchangeable head
Pipetting tips	96 racked filtered tips : 50µl, 200 µl
Accuracy	Single dispense (50µl tips; dry_well transfer):	5µl from 1.5 mL Microtubes (Trueness < 6% ; CV < 6 %)
		10μl from 1.5 mL Microtubes (Trueness < 5% ; CV < 3 %)
		10 μl from easyMAG vessels (Trueness < 1% ; CV < 3 %)
	Single dispense (200µl tips; dry_well transfer):	180 μl from 1.5 mL Microtubes (Trueness < 2% ; CV < 1 %)
	Multi dispenses (200µl tips; dry_well transfer):	15 μl from ARGENE Master mix tubes (Trueness < 1% ; CV < 3 %) (specific pre-characterized Sarstedt tubes)
Master mix on board	Up to 3 x 8 cooled reagents/control tube positions. For ARGENE assays or similar, up to 3 quantitative assays, up to 6 qualitative assays
Time to Result (minutes)	35 min (depending of the number of master mixes used)
Walkaway Time in Minutes	30 min
On-board Sample Capacity	96 eluates
Add Samples with no Pause	No
Minimum / Maximum Sample Volume	From 15 to 1500 µl
Type of Tubes accepted	easyMAG vessels, 0,2 to 1.5 mL microtubes
Dead Volume	easyMAG vessels: < 10µl
	master mix Simport tubes: < 40µl for multidispense
	QS Simport tubes: <15 µl
	Pre-characterized & approved microtubes 1.5 ml: < 15 µl
STAT Handling Method	No
Carryover/Contamination Prevention	Force Tips change option
	HEPA Filter
	UV Lamp
Sample/PCR mixes Clot Detection	Not applicable (eluate)
Sample Liquid Level Sensing	Yes
On-board Reagent Refrigeration	No – Cooling blocks available for certain reagent vessels and PCR microplates
Auto Calibration	No
Software Inventory Management	No
Automatic Maintenance Procedure	No
Daily Maintenance (min)	< 10 min
Technical Support via Modem or Intranet	Possible (Vilink)
Remote Diagnostics	No
On-Line User's Manual	Yes (bioMérieux technical library)