Scientific Posters

The objective is to validate one rapid detection method of spoilage microorganisms, including molds, yeasts, lactic acid and acetic acid bacteria in low pH fruit juice ingredients

The bioMérieux method D COUNT ® 50 based on Flow Cytometry technology, allowed a significant reduction of time to results from 6 days to 3 days providing valid and equivalent results to the reference cultural method (International Fruit and Vegetable Juice Association IFU 2 C).

The chocolate industry is particularly concerned by Salmonella spp. contaminations in raw materials derived from cocoa beans. Salmonella is ubiquitous and present in the environment and can persist in cocoa products along the process of cocoa beans transformation.

Salmonella infection is characterized by acute gastroenteritis. Symptoms include diarrhea, fever, abdominal cramps, and vomiting lasting 4‑7 days in most people.

When present in cocoa products, Salmonella strains are subjected to a high level of stress and can be injured, making complex the detection by conventional and rapid methods. Moreover, the need of a rapid method in one shift is important due to specific manufacturing constraints.

For this reason, bioMérieux has developed a new protocol to reduce significantly the timeto-result from 24 hours for current GENE-UP® SLM2 alternative method to 12 hours for 375g raw materials for chocolate industries.

Detection of Listeria monocytogenes in food and environmental samples following ISO 11290-1:2017 includes sample preparation step using 1/10 dilution followed by secondary enrichment and isolation, requiring four days to release negative results...

Rapid methods such as PCR have been making in-roads into the routine nutraceutical and dietary supplements testing, the potential of multiplex PCR for routine detection of multiple pathogens has yet to be ascertained. The diversity and ever-growing list of matrices in these industries further exacerbates the challenges for such methods and thereby the method compatibility should be reviewed with the adoption of new technology.

The use of traceable culture collection strains, including pathogenic ones, is necessary in food as well as water and environmental laboratories for various applications, including:

• Daily or regular Quality Control (QC) of methods, mandatory for accredited labs

• Performance testing of culture media and reagents

• Verification and in-house validation of methods (e.g., according to ISO 16140-3)

However, the use of (pathogenic) strains, which often require culture and inoculum preparations, can lead to cross-contamination of routine samples.

According to ISO/IEC 17025, testing laboratories should ensure they operate competently and are able to generate valid results. Implementation of Quality Control or Verification of methods requires the use of strains. Genetically Modified Microorganisms (GMM) (e.g., GFP tagged organisms) offer a less risky approach minimizing the impact of cross contamination events securing laboratory results.

Standard molecular methods (without immunoconcentration step) of STEC detection in food matrices based on stx and eae genes co-detection and Top5/7 serogroup associated genes may lead to identify a significant number of false positives without detection of all enterohemorrhagic Escherichia coli (EHEC) potentially present. These major limits may cause either a substantial economic loss for food industry and a food safety risk for consumers.

In the United States, the production and consumption of green leafy produce has been steadily rising. The most recent attribution estimates from Centers for Disease Control and Prevention (2013) indicated leafy produce to be responsible for the highest amount of foodborne illnesses and among the top five causes of foodborne hospitalizations and deaths. Between 1973 and 2012, Salmonella and Shiga toxinproducing Escherichia coli (STEC) species were the top bacterial pathogens responsible for outbreaks caused by leafy vegetables.

In order to meet the growing expectations of consumers and the vigilance of regulation authorities, the food industry now uses new technology to improve food traceability.

Standard molecular methods (without immunoconcentration step) of STEC detection in food matrices based on stx and eae genes co-detection and Top5/7 serogroup associated genes may lead to identify a significant number of false positives without detection of all enterohemorrhagic Escherichia coli (EHEC) potentially present. These major limits may cause either a substantial economic loss for food industry and a food safety risk for consumers.